Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
D-ribose-5-phosphate | at pH 7.5 and 50°C | Geobacillus stearothermophilus | |
0.2 | - |
D-ribose-5-phosphate | at pH 7.5 and 25°C | Geobacillus stearothermophilus | |
5.2 | - |
L-erythrulose | at pH 7.5 and 25°C | Geobacillus stearothermophilus | |
8 | - |
L-erythrulose | at pH 7.5 and 50°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose-5-phosphate + D-ribose-5-phosphate | Geobacillus stearothermophilus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | A0A0I9QGZ2 | formerly Bacillus stearothermophilus | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
50°C, 7 days, no loss of activity | Geobacillus stearothermophilus |
65°C, 3 days, no loss of activity | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanal + hydroxypyruvate | - |
Geobacillus stearothermophilus | 1,3-dihydroxyhexan-2-one + CO2 | - |
? | |
butyraldehyde + hydroxypyruvate | - |
Geobacillus stearothermophilus | ? + CO2 | - |
? | |
D-glyceraldehyde + hydroxypyruvate | - |
Geobacillus stearothermophilus | D-xylulose + CO2 | - |
? | |
D-ribose + hydroxypyruvate | - |
Geobacillus stearothermophilus | ? + CO2 | - |
? | |
D-xylulose-5-phosphate + D-ribose-5-phosphate | - |
Geobacillus stearothermophilus | ? | - |
? | |
DL-glyceraldehyde + hydroxypyruvate | - |
Geobacillus stearothermophilus | ? + CO2 | - |
? | |
glycolaldehyde + hydroxypyruvate | - |
Geobacillus stearothermophilus | L-erythrulose + CO2 | - |
? | |
L-erythrulose + D-ribose-5-phosphate | - |
Geobacillus stearothermophilus | D-sedoheptulose 7-phosphate + glycolaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | more than 50% activity from 40 to 70°C with a sharp decrease thereafter leaving about 25% and 0% activity at 75°C and 85°C, respectively | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 85 | the enzyme shows a half-life of 35 min at 50°C, 15 min at 75°C, and is immediately inactivated at 85°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | more than 50% activity between pH 5.5 and 8.5 | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Geobacillus stearothermophilus |